Coding

Part:BBa_Y00073:Design

Designed by: Natalie Kuldell   Group: Yeast-1   (2006-06-22)


S. pombe gene SPCC126.04c, coded for S. cerevisiae expression


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 1066
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal SpeI site found at 1067
    Illegal PstI site found at 1081
    Illegal NotI site found at 15
    Illegal NotI site found at 1074
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 1067
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal SpeI site found at 1067
    Illegal PstI site found at 1081
    Illegal AgeI site found at 372
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

codon optimization for expression in new species


Source

S. pombe gene recoded for S. cerevisiae expression

References